EzWayTM ECL Western Blot Substrate Kit is an enhanced chemiluminescent substrate with high sensitivity and unique chemiluminescent system. With low background, good stability, sensitive and enhanced signal, this reagent is good for detecting direct and indirect conjugated HRP antibodies and their related antigens, it can detect the target protein sensitively. In the presence of HRP and a peroxide buffer, luminol oxidizes and produces a weak flash of light at 425 nm. Catalyzed by HRP, this substrate can be used to expose X-ray film, and do detection fluorescent CCD scan. |